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SFB 1036 (Heidelberg University)
Repository Table R3 Phosphoproteomics IMAC

This file is part of "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]".

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File Citation
Haneke, Katharina; Stoecklin, Georg, 2020, "Repository Table R3 Phosphoproteomics IMAC", CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4],, heiDATA, V1, UNF:6:JfKZnmkORxFSPz0dUd0TcQ== [fileUNF]
Dataset Citation
Haneke, Katharina; Stoecklin, Georg, 2020, "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]",, heiDATA, V1, UNF:6:+D39RBZQyWVzfn6Sv1nj+w== [fileUNF]
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HeLa cells were SILAC-labeled and treated with DMSO (light) or Ro3306 (heavy, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge precipitation. Phosphopeptides were enriched using PhosSelect iron affinity gel IMAC beads, fractionated and analyzed by mass spectrometry followed by MaxQuant analysis.
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