SFB 1036

Data publications from SFB 1036.

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1 to 10 of 27 Results

CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] Feb 6, 2020 Haneke, Katharina; Stoecklin, Georg, 2020, "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]", https://doi.org/10.11588/data/EFHOBZ, heiDATA, V1, UNF:6:+D39RBZQyWVzfn6Sv1nj+w== [fileUNF] Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling the two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based on the formation of stress granules in human cells, revealed cell cycle-assoc...
Repository Table R1 SG Screen.tab.tab Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] Tab-Delimited - 41.1 KB - MD5: ddd89194e309a7f6e829ed19439d4671 Data The assembly of stress granules (SGs) was monitored in HeLa cells stably expressing GFP-G3BP1 following knock-down of 711 kinases an 256 phosphatases using the Dharmacon siGenome siRNA libraries GU-003505 Human Protein Kinase and GU-003705 Human Protein Phosphatase. Cells were tr...
Repository Table R2 SG Screen Candidates.tab Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] Tab-Delimited - 4.2 KB - MD5: 2cafa8b35d879c452a14511b9e1d575c Data The assembly of stress granules (SGs) was monitored in HeLa cells stably expressing GFP-G3BP1 following knock-down of 711 kinases an 256 phosphatases using the Dharmacon siGenome siRNA libraries GU-003505 Human Protein Kinase and GU-003705 Human Protein Phosphatase. Cells were tr...
Repository Table R3 Phosphoproteomics IMAC n1.tab Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] Tab-Delimited - 453.7 KB - MD5: 157a53ed1a8bf99eae81359b9cc5300c Data HeLa cells were SILAC-labeled and treated with DMSO (light) or Ro3306 (heavy, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge p...
Repository Table R4 Phosphoproteomics TiO2 n2.tab Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] Tab-Delimited - 43.3 KB - MD5: fbfbb3f2f6dd4732bcb176a68fdfc6a7 Data HeLa cells were SILAC-labeled and treated with DMSO (heavy) or Ro3306 (light, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge p...
Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] Mar 5, 2018 - KnopLab Kats, Ilia; Khmelinskii, Anton; Kschonsak, Marc; Huber, Florian; Knieß, Robert A.; Bartosik, Anna; Knop, Michael, 2018, "Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]", https://doi.org/10.11588/data/9ZQIKF, heiDATA, V1 Data accompanying the paper "Mapping degradation signals and pathways in a eukaryotic N-terminome".
N-terminome_rep1.json Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] JSON - 507.5 KB - MD5: b0916e5a056c856b54e317f4c9a5cd20 N-terminome library replicate 1 MPS description file
N-terminome_rep1_run1_1_sequence.txt.gz Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] Gzip Archive - 1.5 GB - MD5: d2096605e24299664b9475f520c42a03 N-terminome library replicate 1, MiSeq run 1, forward read
N-terminome_rep1_run1_2_sequence.txt.gz Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] Gzip Archive - 1.6 GB - MD5: 9d9ee5ae87ed901de6733df32ac5c50a N-terminome library replicate 1, MiSeq run 1, reverse read
N-terminome_rep1_run2_1_sequence.txt.gz Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] Gzip Archive - 2.0 GB - MD5: 20fa92bf009505449855ed122c967d1a N-terminome library replicate 1, MiSeq run 2, forward read

CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Feb 6, 2020

Haneke, Katharina; Stoecklin, Georg, 2020, "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]", https://doi.org/10.11588/data/EFHOBZ, heiDATA, V1, UNF:6:+D39RBZQyWVzfn6Sv1nj+w== [fileUNF]

Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling the two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based on the formation of stress granules in human cells, revealed cell cycle-assoc...

Repository Table R1 SG Screen.tab.tab

Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Tab-Delimited - 41.1 KB - MD5: ddd89194e309a7f6e829ed19439d4671

Data

The assembly of stress granules (SGs) was monitored in HeLa cells stably expressing GFP-G3BP1 following knock-down of 711 kinases an 256 phosphatases using the Dharmacon siGenome siRNA libraries GU-003505 Human Protein Kinase and GU-003705 Human Protein Phosphatase. Cells were tr...

Repository Table R2 SG Screen Candidates.tab

Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Tab-Delimited - 4.2 KB - MD5: 2cafa8b35d879c452a14511b9e1d575c

Data

Repository Table R3 Phosphoproteomics IMAC n1.tab

Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Tab-Delimited - 453.7 KB - MD5: 157a53ed1a8bf99eae81359b9cc5300c

Data

HeLa cells were SILAC-labeled and treated with DMSO (light) or Ro3306 (heavy, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge p...

Repository Table R4 Phosphoproteomics TiO2 n2.tab

Feb 6, 2020 - CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Tab-Delimited - 43.3 KB - MD5: fbfbb3f2f6dd4732bcb176a68fdfc6a7

Data

HeLa cells were SILAC-labeled and treated with DMSO (heavy) or Ro3306 (light, 10 µM) for 4 h. After lysis and disassembly of polysomes in low magnesium buffer, samples were mixed, and ribosomal fractions obtained by sucrose density centrifugation were subjected to Wessel-Flügge p...

Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]

Mar 5, 2018 - KnopLab

Kats, Ilia; Khmelinskii, Anton; Kschonsak, Marc; Huber, Florian; Knieß, Robert A.; Bartosik, Anna; Knop, Michael, 2018, "Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]", https://doi.org/10.11588/data/9ZQIKF, heiDATA, V1

Data accompanying the paper "Mapping degradation signals and pathways in a eukaryotic N-terminome".

N-terminome_rep1.json

Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]

JSON - 507.5 KB - MD5: b0916e5a056c856b54e317f4c9a5cd20

N-terminome library replicate 1 MPS description file

N-terminome_rep1_run1_1_sequence.txt.gz

Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]

Gzip Archive - 1.5 GB - MD5: d2096605e24299664b9475f520c42a03

N-terminome library replicate 1, MiSeq run 1, forward read

N-terminome_rep1_run1_2_sequence.txt.gz

Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]

Gzip Archive - 1.6 GB - MD5: 9d9ee5ae87ed901de6733df32ac5c50a

N-terminome library replicate 1, MiSeq run 1, reverse read

N-terminome_rep1_run2_1_sequence.txt.gz

Mar 5, 2018 - Mapping degradation signals and pathways in a eukaryotic N-terminome [data set]

Gzip Archive - 2.0 GB - MD5: 20fa92bf009505449855ed122c967d1a

N-terminome library replicate 1, MiSeq run 2, forward read

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