Persistent Identifier
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doi:10.11588/data/QDOC4E |
Publication Date
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2021-12-03 |
Title
| Cellular development and evolution of the mammalian cerebellum [Research Data] |
Author
| Sepp, Mari (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Leiss, Kevin (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Sarropoulos, Ioannis (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Murat, Florent (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Okonechnikov, Konstantin (Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany)
Joshi, Piyush (Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany)
Leushkin, Evgeny (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Mbengue, Noe (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Schneider, Céline (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Schmidt, Julia (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Trost, Nils (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Spänig, Lisa (Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany)
Giere, Peter (Museum für Naturkunde Berlin, Leibniz Institute for Research on Evolution and Biodiversity Science at the Humboldt University Berlin, Berlin, Germany)
Khaitovich, Philipp (Center for Neurobiology and Brain Restoration, Skolkovo Institute of Science and Technology, Moscow, Russia)
Lisgo, Steven (Biosciences Institute, Newcastle University, Newcastle, UK)
Palkovits, Miklós (Human Brain Tissue Bank, Semmelweis University, Budapest, Hungary)
Kutscher, Lena M. (Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany)
Anders, Simon (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Cardoso-Moreira, Margarida (Evolutionary Developmental Biology Laboratory, Francis Crick Institute, London NW1 1AT, UK)
Pfister, Stefan M. (Hopp-Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany)
Kaessmann, Henrik (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH)) |
Point of Contact
|
Use email button above to contact.
Kaessmann, Henrik (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Leiss, Kevin (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH))
Sepp, Mari (Heidelberg University, Center for Molecular Biology of Heidelberg University (ZMBH)) |
Description
| The expansion of the neocortex, one of the hallmarks of mammalian evolution was accompanied by an increase in the number of cerebellar neurons. However, little is known about the evolution of the cellular programs underlying cerebellum development in mammals. In this study, we generated single-nucleus RNA-sequencing data for ~400,000 cells to trace the development of the cerebellum from early neurogenesis to adulthood in human, mouse, and the marsupial opossum. Our cross-species analyses revealed that the cellular composition and differentiation dynamics throughout cerebellum development are largely conserved, except for human Purkinje cells. Global transcriptome profiles, conserved cell state markers, and gene expression trajectories across neuronal differentiation show that the cerebellar cell type-defining programs have been preserved for at least 160 million years. However, we also discovered differences. We identified 3,586 genes that either gained or lost expression in cerebellar cells in one of the species, and 541 genes that evolved new expression trajectories during neuronal differentiation. The potential functional relevance of these cross-species differences is highlighted by the diverged expression patterns of several human disease-associated genes. Altogether, our study reveals shared and lineage-specific programs governing the cellular development of the mammalian cerebellum, and expands our understanding of the evolution of mammalian organ development. |
Subject
| Medicine, Health and Life Sciences |
Keyword
| single nucleus RNA-seq, cerebellum, development, evolution, transcriptomics |
Topic Classification
| evolution, development, neuroscience |
Funding Information
| European Research Council: 615253, OntoTransEvol
European Research Council: 819894, BRAIN-MATCH
Brain Research Program: 2017-1.2.1-NKP-2017-00002
Cancer Research UK: FC011171
UK Medical Research Council: FC011171
Wellcome Trust: FC011171 |
Data Type
| single nucleus transcripomes |