Persistent Identifier
|
doi:10.11588/data/N8NHE2 |
Publication Date
|
2017-12-04 |
Title
| Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset] |
Author
| Hildenbrand, Georg (Department of Radiation Oncology, University Medical Centre Mannheim)
Metzler, Philipp (Kirchhoff-Institute for Physics, Heidelberg University)
Pilarczyk, Goetz (Kirchhoff-Institute for Physics, Heidelberg University)
Bobu, Vladimir (Department of Radiation Oncology, University Medical Centre Mannheim)
Hosser, Hiltraud (Department of Radiation Oncology, University Medical Centre Mannheim)
Fleckenstein, Jens (Department of Radiation Oncology, University Medical Centre Mannheim)
Krufczik, Matthias (Kirchhoff-Institute for Physics, Heidelberg University)
Bestvater, Felix (German Cancer Research Center (DKFZ), Heidelberg)
Wenz, Frederik (Department of Radiation Oncology, University Medical Centre Mannheim)
Hausmann, Michael (Kirchhoff-Institute for Physics, Heidelberg University) |
Point of Contact
|
Use email button above to contact.
Hausmann, Michael (Kirchhoff-Institute for Physics, Heidelberg University) |
Description
| Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles (“SmartFlares”) can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both – SmartFlares and unmodified GNPs – lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles. |
Subject
| Medicine, Health and Life Sciences; Physics |
Related Publication
| Hildenbrand G, Metzler P, Pilarczyk G, Bobu V, Kriz W, et al. (2018) Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells. PLOS ONE 13(1): e0190183. doi: 10.1371/journal.pone.0190183 https://doi.org/10.1371/journal.pone.0190183
Moser F, Hildenbrand G, Müller P, Al Saroori A, Biswas A, Bach M, Wenz F, Cremer C, Burger N, Veldwijk M, Hausmann M (2016) Cellular uptake of gold nanoparticles and their behavior as labels for localization microscopy. Biophys J 110: 947-953 doi: 10.1016/j.bpj.2016.01.004 https://doi.org/10.1016/j.bpj.2016.01.004
Burger N, Biswas A, Barzan D, Kirchner A, Hosser H, Hausmann M, Hildenbrand G, Herskind C, Wenz F, Veldwijk MR (2014) A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells. Nanomedicine: NBM 10: 1365-1373 doi: 10.1016/j.nano.2014.03.011 https://doi.org/10.1016/j.nano.2014.03.011 |
Notes
| .nd2-files can be read e.g. via NIS-Elements Viewer |
Language
| English |
Deposit Date
| 2017-11-29 |