Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]  (ICPSR doi:10.11588/data/N8NHE2)

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Document Description

Citation

Title:

Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]

Identification Number:

doi:10.11588/data/N8NHE2

Distributor:

heiDATA

Date of Distribution:

2017-12-04

Version:

1

Bibliographic Citation:

Hildenbrand, Georg; Metzler, Philipp; Pilarczyk, Goetz; Bobu, Vladimir; Hosser, Hiltraud; Fleckenstein, Jens; Krufczik, Matthias; Bestvater, Felix; Wenz, Frederik; Hausmann, Michael, 2017, "Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]", https://doi.org/10.11588/data/N8NHE2, heiDATA, V1

Study Description

Citation

Title:

Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]

Identification Number:

doi:10.11588/data/N8NHE2

Authoring Entity:

Hildenbrand, Georg (Department of Radiation Oncology, University Medical Centre Mannheim)

Metzler, Philipp (Kirchhoff-Institute for Physics, Heidelberg University)

Pilarczyk, Goetz (Kirchhoff-Institute for Physics, Heidelberg University)

Bobu, Vladimir (Department of Radiation Oncology, University Medical Centre Mannheim)

Hosser, Hiltraud (Department of Radiation Oncology, University Medical Centre Mannheim)

Fleckenstein, Jens (Department of Radiation Oncology, University Medical Centre Mannheim)

Krufczik, Matthias (Kirchhoff-Institute for Physics, Heidelberg University)

Bestvater, Felix (German Cancer Research Center (DKFZ), Heidelberg)

Wenz, Frederik (Department of Radiation Oncology, University Medical Centre Mannheim)

Hausmann, Michael (Kirchhoff-Institute for Physics, Heidelberg University)

Distributor:

heiDATA

Date of Distribution:

2017-12-04

Study Scope

Keywords:

Medicine, Health and Life Sciences, Physics

Abstract:

Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles (“SmartFlares”) can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both – SmartFlares and unmodified GNPs – lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles.

Notes:

.nd2-files can be read e.g. via <a href="https://www.nikoninstruments.com/en_DE/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer">NIS-Elements Viewer</a>

Methodology and Processing

Other Study-Related Materials

Label:

Foci_Analysis.zip

Text:

nd2-files [can be read e.g. via NIS-Elements Viewer (https://www.nikoninstruments.com/en_DE/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer)]

Notes:

application/zip

Other Study-Related Materials

Label:

Localization_Microscopy.zip

Text:

png files

Notes:

application/zip