CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] (ICPSR doi:10.11588/data/EFHOBZ)

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Part 1: Document Description
Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Entire Codebook

Document Description

Citation

Title:

CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Identification Number:

doi:10.11588/data/EFHOBZ

Distributor:

heiDATA

Date of Distribution:

2020-02-06

Version:

1

Bibliographic Citation:

Haneke, Katharina; Stoecklin, Georg, 2020, "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]", https://doi.org/10.11588/data/EFHOBZ, heiDATA, V1, UNF:6:+D39RBZQyWVzfn6Sv1nj+w== [fileUNF]

Study Description

Citation

Title:

CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]

Identification Number:

doi:10.11588/data/EFHOBZ

Authoring Entity:

Haneke, Katharina (Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Germany)

Stoecklin, Georg (Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Germany)

Producer:

Division of Biochemistry, Medical Faculty Mannheim

Grant Number:

SFB1036, 201348542

Grant Number:

TRR186, 278001972

Distributor:

heiDATA

Date of Distribution:

2020-02-06

Study Scope

Keywords:

Medicine, Health and Life Sciences

Abstract:

Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling the two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based on the formation of stress granules in human cells, revealed cell cycle-associated kinases as major candidates. CDK1 was identified as a positive regulator of global translation, and cell synchronization experiments showed that this is an extra-mitotic function of CDK1. Different pathways including eIF2α, 4EBP1 and S6K1 signaling contribute to controlling global translation downstream of CDK1. Moreover, Ribo-Seq analysis uncovered that CDK1 exerts a particularly strong effect on the translation of 5’TOP mRNAs, which includes mRNAs encoding for ribosomal proteins and several translation factors. This effect requires the 5’TOP mRNA-binding protein LARP1, concurrent to our finding that LARP1 phosphorylation is strongly dependent on CDK1. Thus, CDK1 provides a direct means to couple cell proliferation with biosynthesis of the translation machinery and the rate of protein synthesis.

Methodology and Processing

File Description--f3297

File: Repository Table R1 SG Screen.tab.tab

  • Number of cases: 986

  • No. of variables per record: 9

  • Type of File: text/tab-separated-values

Notes:

UNF:6:iGFGYLz0Wtn7xM/H53+5jw==

File Description--f3153

File: Repository Table R2 SG Screen Candidates.tab

  • Number of cases: 71

  • No. of variables per record: 14

  • Type of File: text/tab-separated-values

Notes:

UNF:6:J05CBEaCX/BstRp7yZfDBA==

File Description--f3154

File: Repository Table R3 Phosphoproteomics IMAC n1.tab

  • Number of cases: 2918

  • No. of variables per record: 9

  • Type of File: text/tab-separated-values

Notes:

UNF:6:JfKZnmkORxFSPz0dUd0TcQ==

File Description--f3155

File: Repository Table R4 Phosphoproteomics TiO2 n2.tab

  • Number of cases: 273

  • No. of variables per record: 9

  • Type of File: text/tab-separated-values

Notes:

UNF:6:TuQsCZsNr94F55djEF3jlA==

Variable Description

List of Variables:

Variables

Plate

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Variable Format: character

Notes: UNF:6:CE83FurSKiZWKCSKH8dIfg==

Well

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Variable Format: character

Notes: UNF:6:iphnr8GamVPVrkc786WpZA==

Genesymbol

f3297 Location:

Variable Format: character

Notes: UNF:6:Tl1tEiiWwFgK6yMp0Hv3Vw==

siRNA

f3297 Location:

Variable Format: character

Notes: UNF:6:yYWIHWlBV6amvG7Yd9BhiQ==

siRNA2

f3297 Location:

Variable Format: character

Notes: UNF:6:VEqyrZ8/k6tbnWzzY9SZ7A==

siRNA3

f3297 Location:

Variable Format: character

Notes: UNF:6:rNLVFr8RvZmzieCtguObbw==

siRNA4

f3297 Location:

Variable Format: character

Notes: UNF:6:pzk/HLZ0iXs0zn55GT/p+A==

product

f3297 Location:

Variable Format: character

Notes: UNF:6:hieHIQz0Dc4HcQTT6zGdlg==

replicates

f3297 Location:

Variable Format: character

Notes: UNF:6:a16F/onBvL5SY5NIn9kg5A==

Plate

f3153 Location:

Variable Format: character

Notes: UNF:6:2b80Qr9GHUunlMDYpBKmlg==

Well

f3153 Location:

Variable Format: character

Notes: UNF:6:JkHEJYMXbNeQ8gZlT9du/g==

Genesymbol

f3153 Location:

Variable Format: character

Notes: UNF:6:Ixm8c5ektNfFpDhggJNJhQ==

dx1

f3153 Location:

Variable Format: character

Notes: UNF:6:X2kq3cOvi9hKE1+5ccie2Q==

dx2

f3153 Location:

Variable Format: character

Notes: UNF:6:9vn/mY1rx8N0SUE/HoomqQ==

dx3

f3153 Location:

Variable Format: character

Notes: UNF:6:biMAvXeTjvoIV8aF2SsPPA==

dx4

f3153 Location:

Variable Format: character

Notes: UNF:6:0v5KuEMT+bAzuyGf7yxmjw==

sum

f3153 Location:

Variable Format: character

Notes: UNF:6:DLKlptfjGj3e6tb7Szzz7g==

n

f3153 Location:

Variable Format: character

Notes: UNF:6:BwlwTnj1VLb0D4H+TR5NVQ==

SGscore

f3153 Location:

Variable Format: character

Notes: UNF:6:dXe7BPwq/ZJvB6h1BW9T+g==

A

f3153 Location:

Variable Format: character

Notes: UNF:6:FX/emqwUzAOrbF1pK64FNA==

B

f3153 Location:

Variable Format: character

Notes: UNF:6:RmNHcHppi3kP8rbzezd5gg==

C

f3153 Location:

Variable Format: character

Notes: UNF:6:Pe6miuFwMXya311B0e9G5g==

D

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Variable Format: character

Notes: UNF:6:4l3ZqrQE0i3pqjYg3K2U1w==

Gene.names

f3154 Location:

Variable Format: character

Notes: UNF:6:AV1NyqBNYGDt9TH8dYWrKw==

DeltaPhosphoproteomics

f3154 Location:

Summary Statistics: Min. -6.60908; Valid 2918.0; Max. 4.83683; Mean -0.38012580336977386; StDev 0.7298382755284031;

Variable Format: numeric

Notes: UNF:6:4zNe2HZvzbdhwkEZifB2MQ==

DeltaProteomics

f3154 Location:

Summary Statistics: Valid 2221.0; StDev 0.39561945432237666; Mean -0.22106292122374; Min. -1.75015; Max. 1.74149;

Variable Format: numeric

Notes: UNF:6:FKMWMXCbGrQdTqOjbiGOrA==

Positions

f3154 Location:

Summary Statistics: Valid 2918.0; Mean 544.356408498969; Max. 6753.0; StDev 682.2369037061802; Min. 2.0

Variable Format: numeric

Notes: UNF:6:DF+MkqHsiSvKO1KIShKieQ==

Sequencewindow

f3154 Location:

Variable Format: character

Notes: UNF:6:lXKkew6Q7CjwLW2i81wT2g==

Proteins

f3154 Location:

Variable Format: character

Notes: UNF:6:zrCjQMh1de1cxQJAU1MLIw==

Leading.proteins

f3154 Location:

Variable Format: character

Notes: UNF:6:Dm0J1omjL5BfrZXca6q25w==

Protein

f3154 Location:

Variable Format: character

Notes: UNF:6:n8e8XwijErFqcKcbSXIsvw==

Protein.names

f3154 Location:

Variable Format: character

Notes: UNF:6:zySxQA5suzwMNIEk/ms1eg==

Gene.names

f3155 Location:

Variable Format: character

Notes: UNF:6:iEPuokut3e4/Kxc6z04EqQ==

DeltaPhosphoproteomics

f3155 Location:

Summary Statistics: Valid 223.0; Max. 5.58755012944285; StDev 0.7855580404106924; Mean -0.15264262371667983; Min. -2.42166765492975

Variable Format: numeric

Notes: UNF:6:J5Bafkl8GkKu90wrVyHS8w==

DeltaProteomics

f3155 Location:

Summary Statistics: Max. 0.573724509002663; StDev 0.24921960581747996; Mean 0.047936784842966165; Valid 93.0; Min. -1.15276751926177

Variable Format: numeric

Notes: UNF:6:09OqScMnE04MrUuLTjv/Nw==

Positions

f3155 Location:

Variable Format: character

Notes: UNF:6:Wm6JpD4NNu1eTQHZH7GZcA==

Sequence.window

f3155 Location:

Variable Format: character

Notes: UNF:6:RCLN5dAby7+2zG2nQhnT6Q==

Proteins

f3155 Location:

Variable Format: character

Notes: UNF:6:OJP/LspnxE0YXjpJNdWffw==

Leading.proteins

f3155 Location:

Variable Format: character

Notes: UNF:6:4qnemttR4QrAwHhGo5L1zw==

Protein

f3155 Location:

Variable Format: character

Notes: UNF:6:46VsFgf7CABeESorIQT4Ew==

Protein.names

f3155 Location:

Variable Format: character

Notes: UNF:6:WVOWaRgKMRpYq8KTDeh1ng==