SPDM data capturing radiation induced chromatin conformation changes (ICPSR doi:10.11588/data/10031)

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Document Description

Citation

Title:

SPDM data capturing radiation induced chromatin conformation changes

Identification Number:

doi:10.11588/data/10031

Distributor:

heiDATA

Date of Distribution:

2015-05-19

Version:

3

Bibliographic Citation:

Hausmann, Michael; Müller, Patrick; Hillebrandt, Sabina; Bach, Margund; Kaufmann, Rainer; Zhang, Yang, 2015, "SPDM data capturing radiation induced chromatin conformation changes", https://doi.org/10.11588/data/10031, heiDATA, V3

Study Description

Citation

Title:

SPDM data capturing radiation induced chromatin conformation changes

Identification Number:

doi:10.11588/data/10031

Authoring Entity:

Hausmann, Michael (Kirchhoff-Institute for Physics)

Müller, Patrick (Kirchhoff-Institute for Physics)

Hillebrandt, Sabina (Kirchhoff-Institute for Physics)

Bach, Margund (Kirchhoff-Institute for Physics)

Kaufmann, Rainer (Kirchhoff-Institute for Physics)

Zhang, Yang (Kirchhoff-Institute for Physics)

Producer:

Hausmann, Michael

Müller, Patrick

Hillebrandt, Sabina

Bach, Margund

Kaufmann, Rainer

Date of Production:

2014-10-14

Grant Number:

FKZ: SR/StSch/INT 3610S30015

Distributor:

heiDATA

Date of Distribution:

2015-05-19

Study Scope

Keywords:

Medicine, Health and Life Sciences, Physics

Abstract:

<p>Using stably transfected HeLa cells expressing either green fluorescent protein (GFP) labelled histone H2B or yellow fluorescent protein (YFP) labelled histone H2A, we investigated the positioning of individual histone proteins in cell nuclei by means of high resolution localization microscopy (Spectral Position Determination Microscopy = SPDM). The cells were exposed to ionizing radiation of different doses and aliquots were fixed after different repair times for SPDM imaging. In addition to the repair dependent histone protein pattern, the positioning of antibodies specific for heterochromatin and euchromatin was recorded by SPDM. </p> <p>Experimental data was acquired in the Experimental Biophysics group by Michael Hausmann, Patrick Müller, Sabina Hillebrandt, Margund Bach and Rainer Kaufmann.</p> <p>Kernel Density Estimations of the experimental data and the maskings of the regions of interest based on the KDEs were calculated by Yang Zhang, a member of the Statistical Physics and Theoretical Biophysics Group.</p>

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