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Part 1: Document Description
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Citation |
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Title: |
Rapid On-Resin N-Formylation of Peptides as One-Pot Reaction [Research Data] |
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Identification Number: |
doi:10.11588/data/00RULE |
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Distributor: |
heiDATA |
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Date of Distribution: |
2024-02-09 |
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Version: |
1 |
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Bibliographic Citation: |
Kokollari, Agon; Werner, Marius; Lindner, Christina; Pham, Truc Lam; Thomas, Franziska, 2024, "Rapid On-Resin N-Formylation of Peptides as One-Pot Reaction [Research Data]", https://doi.org/10.11588/data/00RULE, heiDATA, V1 |
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Citation |
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Title: |
Rapid On-Resin N-Formylation of Peptides as One-Pot Reaction [Research Data] |
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Identification Number: |
doi:10.11588/data/00RULE |
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Authoring Entity: |
Kokollari, Agon (Heidelberg University) |
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Werner, Marius (Heidelberg University) |
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Lindner, Christina (Heidelberg University) |
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Pham, Truc Lam (Heidelberg University) |
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Thomas, Franziska (Heidelberg University) |
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Grant Number: |
414261058, 2082/1–390761711 (3DMM2O) |
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Grant Number: |
Kekulé Fellowship (T.L.P) |
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Distributor: |
heiDATA |
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Access Authority: |
Thomas, Franziska |
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Access Authority: |
Pham, Truc Lam |
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Date of Deposit: |
2024-01-31 |
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Holdings Information: |
https://doi.org/10.11588/data/00RULE |
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Study Scope |
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Keywords: |
Chemistry, chemotactic peptides, late-stage formylation, post-translational modification, protein modifications, solid-phase synthesis |
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Abstract: |
N-formylation is a common pre- and post-translational modification of the N-terminus or the lysine side chain of peptides and proteins that plays a role in the initiation of immune responses, gene expression, or epigenetics. Despite its high biological relevance, protocols for the chemical N-formylation of synthetic peptides are scarce. The few available methods are elaborate in their execution and the yields are highly sequence-dependent. We present a rapid, easy-to-use one-pot procedure that runs at room temperature and can be used to formylate protected peptides at both the N-terminus and the lysine side chain on the resin in near-quantitative yields. Only insensitive, storage-stable standard chemicals – formic acid, acetic anhydride, pyridine and DMF – are used. Formylation works for both short and long peptides of up to 34 amino acids and over the spectrum of canonical amino acids. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
A. Kokollari, M. Werner, C. Lindner, T. L. Pham, F. Thomas, ChemBioChem 2023, e202300571. |
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Identification Number: |
10.1002/cbic.202300571 |
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Bibliographic Citation: |
A. Kokollari, M. Werner, C. Lindner, T. L. Pham, F. Thomas, ChemBioChem 2023, e202300571. |
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Label: |
1_Optimization_of_P1_formylation.zip |
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Notes: |
application/zip |
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Label: |
2_Formylation_of_P2_to_P6.zip |
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Notes: |
application/zip |
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Label: |
Original_Data_Overview.docx |
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Notes: |
application/vnd.openxmlformats-officedocument.wordprocessingml.document |
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Label: |
Original_Data_Overview.pdf |
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Notes: |
application/pdf |