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Part 1: Document Description
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Citation |
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Title: |
Dissecting intratumor heterogeneity of nodal B cell lymphomas at transcriptional, genetic, and drug response levels |
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Identification Number: |
doi:10.11588/data/VRJUNV |
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Distributor: |
heiDATA |
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Date of Distribution: |
2020-04-06 |
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Version: |
1 |
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Bibliographic Citation: |
Roider, Tobias; Seufert, Julian; Uvarovskii, Alexey; Frauhammer, Felix; Bordas, Marie; Abedpour, Nima; Stolarczyk, Marta; Mallm, Jan-Philipp; Herbst, Sophie A.; Bruch, Peter-Martin; Balke-Want, Hyatt; Hundemer, Michael; Rippe, Karsten; Goeppert, Benjamin; Seiffert, Martina; Brors, Benedikt; Mechtersheimer, Gunhild; Zenz, Thorsten; Peifer, Martin; Chapuy, Björn; Schlesner, Matthias; Müller-Tidow, Carsten; Fröhling, Stefan; Huber, Wolfgang; Anders, Simon; Sascha Dietrich, 2020, "Dissecting intratumor heterogeneity of nodal B cell lymphomas at transcriptional, genetic, and drug response levels", https://doi.org/10.11588/data/VRJUNV, heiDATA, V1, UNF:6:AlKw3YwYZ82Ty5CxnC4D5w== [fileUNF] |
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Citation |
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Title: |
Dissecting intratumor heterogeneity of nodal B cell lymphomas at transcriptional, genetic, and drug response levels |
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Alternative Title: |
scRNA-seq in nodal B cell lymphoma |
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Identification Number: |
doi:10.11588/data/VRJUNV |
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Authoring Entity: |
Roider, Tobias (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Seufert, Julian (Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Uvarovskii, Alexey (Center for Molecular Biology of the University of Heidelberg, Heidelberg, Germany) |
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Frauhammer, Felix (Center for Molecular Biology of the University of Heidelberg, Heidelberg, Germany) |
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Bordas, Marie (Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Abedpour, Nima (University of Cologne, Department for Translational Genomics, Cologne, Germany) |
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Stolarczyk, Marta (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Mallm, Jan-Philipp (Division of Chromatin Networks, German Cancer Research Center (DKFZ) & Bioquant, Heidelberg, Germany) |
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Herbst, Sophie A. (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Bruch, Peter-Martin (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Balke-Want, Hyatt (University of Cologne, Department for Translational Genomics, Cologne, Germany) |
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Hundemer, Michael (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Rippe, Karsten (Division of Chromatin Networks, German Cancer Research Center (DKFZ) & Bioquant, Heidelberg, Germany) |
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Goeppert, Benjamin (Institute of Pathology, University of Heidelberg, Heidelberg, Germany) |
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Seiffert, Martina (Division of Molecular Genetics, German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Brors, Benedikt (Division of Applied Bioinformatics, German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Mechtersheimer, Gunhild (Institute of Pathology, University of Heidelberg, Heidelberg, Germany) |
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Zenz, Thorsten (Department of Medical Oncology and Hematology, University of Zürich, Zürich, Switzerland) |
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Peifer, Martin (University of Cologne, Department for Translational Genomics, Cologne, Germany) |
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Chapuy, Björn (Clinic for Hematology and Medical Oncology, University Medicine Göttingen, Göttingen, Germany) |
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Schlesner, Matthias (Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Müller-Tidow, Carsten (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Fröhling, Stefan (Department of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg and German Cancer Research Center (DKFZ), Heidelberg, Germany) |
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Huber, Wolfgang (European Molecular Biology Laboratory (EMBL), Heidelberg, Germany) |
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Anders, Simon (Center for Molecular Biology of the University of Heidelberg, Heidelberg, Germany) |
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Sascha Dietrich (Department of Medicine V, Hematology, Oncology and Rheumatology, University of Heidelberg, Heidelberg, Germany) |
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Distributor: |
heiDATA |
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Access Authority: |
Roider, Tobias |
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Access Authority: |
Dietrich, Sascha |
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Access Authority: |
Anders, Simon |
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Holdings Information: |
https://doi.org/10.11588/data/VRJUNV |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences |
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Abstract: |
Tumour heterogeneity encompasses both the malignant cells and their microenvironment. While heterogeneity between individual patients is known to affect the efficacy of cancer therapy, most personalized treatment approaches do not account for intratumor heterogeneity. We addressed this issue by studying the heterogeneity of nodal B cell lymphoma by single cell RNA-sequencing and transcriptome-informed flow cytometry. We identified transcriptionally distinct malignant subpopulations and compared their drug response and genomic profiles. Malignant subpopulations of the same patient responded strikingly different to anti-cancer drugs ex vivo, which recapitulated subpopulation-specific drug sensitivity during in vivo treatment. Infiltrating T cells represented the majority of non-malignant cells, whose gene expression signatures were similar across all donors, whereas the frequencies of T cell subsets varied significantly between the donors. Our data provide insights into the heterogeneity of nodal B cell lymphoma and highlight the relevance of intratumor heterogeneity for personalized cancer therapy. |
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Notes: |
10x data processed by CellRanger: sparse-matrix format with raw UMI counts. For detailed format description, see <a href="https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/output/matrices">https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/output/matrices</a>. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Roider, T., Seufert, J., Uvarovskii, A. et al. Dissecting intratumour heterogeneity of nodal B-cell lymphomas at the transcriptional, genetic and drug-response levels. Nat Cell Biol 22, 896–906 (2020). |
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Identification Number: |
https://doi.org/10.1038/s41556-020-0532-x |
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Bibliographic Citation: |
Roider, T., Seufert, J., Uvarovskii, A. et al. Dissecting intratumour heterogeneity of nodal B-cell lymphomas at the transcriptional, genetic and drug-response levels. Nat Cell Biol 22, 896–906 (2020). |
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File Description--f3382 |
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File: AnnotationCells.tab |
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Notes: |
UNF:6:5fjnKQ0MiCiccCbgx/Dyxw== |
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File Description--f3383 |
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File: Annotation_Tcells.tab |
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Notes: |
UNF:6:NUwQFL4G9cwzqMGh4Ab4jA== |
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File Description--f2843 |
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File: sample_sheet.tab |
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Notes: |
UNF:6:dLH9pDgli3I+s+BIDRvtBQ== |
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List of Variables: |
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Variables |
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f3382 Location: |
Variable Format: character Notes: UNF:6:5fjnKQ0MiCiccCbgx/Dyxw== |
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f3383 Location: |
Variable Format: character Notes: UNF:6:NUwQFL4G9cwzqMGh4Ab4jA== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:BAo//803ZVWvc8cyDIX4kw== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:CT+eHHdhTxKjMQq6THXujA== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:vesnBEh9wKLHVFmV2U2+5g== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:evTXaE1XVPxSNunJZXZSfQ== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:ZqK3PZ5lnlaw/7IpQY6syA== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:Y36uGgIEoK0/uCGe7RvY2g== |
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f2843 Location: |
Summary Statistics: Max. 2.0; StDev 0.5149286505444373; Valid 12.0; Mean 1.4166666666666665; Min. 1.0; Variable Format: numeric Notes: UNF:6:5OOy8v37V/5CazRviM2QaQ== |
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f2843 Location: |
Variable Format: character Notes: UNF:6:I4hBoPc0EbFTGTe9TJ8K3A== |
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Label: |
DLBCL1.zip |
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Notes: |
application/zip |
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Label: |
DLBCL2.zip |
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Notes: |
application/zip |
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Label: |
DLBCL3.zip |
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Notes: |
application/zip |
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Label: |
FL1.zip |
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Notes: |
application/zip |
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Label: |
FL2.zip |
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Notes: |
application/zip |
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Label: |
FL3.zip |
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Notes: |
application/zip |
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FL4.zip |
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Notes: |
application/zip |
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Label: |
rLN1.zip |
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Notes: |
application/zip |
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rLN2.zip |
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Notes: |
application/zip |
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Label: |
rLN3.zip |
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Notes: |
application/zip |
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Label: |
tFL1.zip |
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Notes: |
application/zip |
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Label: |
tFL2.zip |
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Notes: |
application/zip |