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Part 1: Document Description
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Citation |
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Title: |
Printing and Erasing of DNA-based Photoresists inside Synthetic Cells [RESEARCH DATA] |
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Identification Number: |
doi:10.11588/data/MKOC9S |
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Distributor: |
heiDATA |
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Date of Distribution: |
2022-02-28 |
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Version: |
1 |
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Bibliographic Citation: |
Goepfrich, Kerstin; Walther, Tobias, 2022, "Printing and Erasing of DNA-based Photoresists inside Synthetic Cells [RESEARCH DATA]", https://doi.org/10.11588/data/MKOC9S, heiDATA, V1 |
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Citation |
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Title: |
Printing and Erasing of DNA-based Photoresists inside Synthetic Cells [RESEARCH DATA] |
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Identification Number: |
doi:10.11588/data/MKOC9S |
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Authoring Entity: |
Goepfrich, Kerstin (Heidelberg University, Cluster of Excellence "3D Matter Made to Order" and Max Planck Institute for Medical Research, Heidelberg, Germany) |
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Walther, Tobias (Heidelberg University, Cluster of Excellence "3D Matter Made to Order" and Max Planck Institute for Medical Research, Heidelberg, Germany) |
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Grant Number: |
EXC-2082/1 - 390761711 |
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Distributor: |
heiDATA |
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Access Authority: |
Goepfrich, Kerstin |
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Holdings Information: |
https://doi.org/10.11588/data/MKOC9S |
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Study Scope |
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Keywords: |
Chemistry, Engineering, Medicine, Health and Life Sciences, Physics |
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Abstract: |
In the pursuit to produce functioning synthetic cells from the bottom up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38um. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology. |
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Methodology and Processing |
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Data Access |
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Other Study Description Materials |
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Figure_1.zip |
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application/zip |
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Figure_2.zip |
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application/zip |
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Figure_3.zip |
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application/zip |
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Figure_4.zip |
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application/zip |
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nupack_melting_temperature.zip |
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application/zip |
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SI_bleaching_control_erasing.zip |
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application/zip |
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SI_bulk_characterization.zip |
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application/zip |
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SI_bulk_linker_concentration.zip |
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application/zip |
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SI_bulk_printing_erasing_stability.zip |
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application/zip |
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SI_Droplet_characterization.zip |
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application/zip |
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SI_Droplet_stability_dextran.zip |
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application/zip |
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SI_GUV_stability.zip |
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application/zip |
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SI_heating.zip |
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application/zip |
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SI_PAGE.zip |
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application/zip |
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SI_Printing_width.zip |
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application/zip |
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SI_Video.zip |
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application/zip |
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ToC.zip |
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application/zip |