|
View: |
Part 1: Document Description
|
|
Citation |
|
|---|---|
|
Title: |
CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] |
|
Identification Number: |
doi:10.11588/data/EFHOBZ |
|
Distributor: |
heiDATA |
|
Date of Distribution: |
2020-02-06 |
|
Version: |
1 |
|
Bibliographic Citation: |
Haneke, Katharina; Stoecklin, Georg, 2020, "CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4]", https://doi.org/10.11588/data/EFHOBZ, heiDATA, V1, UNF:6:+D39RBZQyWVzfn6Sv1nj+w== [fileUNF] |
|
Citation |
|
|
Title: |
CDK1 couples proliferation with protein synthesis [Repository Tables R1-R4] |
|
Identification Number: |
doi:10.11588/data/EFHOBZ |
|
Authoring Entity: |
Haneke, Katharina (Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Germany) |
|
Stoecklin, Georg (Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Germany) |
|
|
Other identifications and acknowledgements: |
Stoecklin, Georg |
|
Producer: |
Division of Biochemistry, Medical Faculty Mannheim |
|
Grant Number: |
SFB1036, 201348542 |
|
Grant Number: |
TRR186, 278001972 |
|
Distributor: |
heiDATA |
|
Access Authority: |
Haneke, Katharina |
|
Depositor: |
Stoecklin, Georg |
|
Date of Deposit: |
2019-11-20 |
|
Holdings Information: |
https://doi.org/10.11588/data/EFHOBZ |
|
Study Scope |
|
|
Keywords: |
Medicine, Health and Life Sciences |
|
Abstract: |
Cell proliferation exerts a high demand on protein synthesis, yet the mechanisms coupling the two processes are not fully understood. A kinase and phosphatase screen for activators of translation, based on the formation of stress granules in human cells, revealed cell cycle-associated kinases as major candidates. CDK1 was identified as a positive regulator of global translation, and cell synchronization experiments showed that this is an extra-mitotic function of CDK1. Different pathways including eIF2α, 4EBP1 and S6K1 signaling contribute to controlling global translation downstream of CDK1. Moreover, Ribo-Seq analysis uncovered that CDK1 exerts a particularly strong effect on the translation of 5’TOP mRNAs, which includes mRNAs encoding for ribosomal proteins and several translation factors. This effect requires the 5’TOP mRNA-binding protein LARP1, concurrent to our finding that LARP1 phosphorylation is strongly dependent on CDK1. Thus, CDK1 provides a direct means to couple cell proliferation with biosynthesis of the translation machinery and the rate of protein synthesis. |
|
Methodology and Processing |
|
|
Sources Statement |
|
|
Data Access |
|
|
Other Study Description Materials |
|
|
Related Publications |
|
|
Citation |
|
|
Title: |
Katharina Haneke, Johanna Schott, Doris Lindner, Anne Kruse Hollensen, Christian Kroun Damgaard, Cyril Mongis, Michael Knop, Wilhelm Palm, Alessia Ruggieri, Georg Stoecklin; CDK1 couples proliferation with protein synthesis. J Cell Biol 2 March 2020; 219 (3): e201906147. |
|
Identification Number: |
https://doi.org/10.1083/jcb.201906147 |
|
Bibliographic Citation: |
Katharina Haneke, Johanna Schott, Doris Lindner, Anne Kruse Hollensen, Christian Kroun Damgaard, Cyril Mongis, Michael Knop, Wilhelm Palm, Alessia Ruggieri, Georg Stoecklin; CDK1 couples proliferation with protein synthesis. J Cell Biol 2 March 2020; 219 (3): e201906147. |
|
File Description--f3297 |
|
|
File: Repository Table R1 SG Screen.tab.tab |
|
|
|
|
Notes: |
UNF:6:iGFGYLz0Wtn7xM/H53+5jw== |
|
File Description--f3153 |
|
|
File: Repository Table R2 SG Screen Candidates.tab |
|
|
|
|
Notes: |
UNF:6:J05CBEaCX/BstRp7yZfDBA== |
|
File Description--f3154 |
|
|
File: Repository Table R3 Phosphoproteomics IMAC n1.tab |
|
|
|
|
Notes: |
UNF:6:JfKZnmkORxFSPz0dUd0TcQ== |
|
File Description--f3155 |
|
|
File: Repository Table R4 Phosphoproteomics TiO2 n2.tab |
|
|
|
|
Notes: |
UNF:6:TuQsCZsNr94F55djEF3jlA== |
|
List of Variables: |
|
|
Variables |
|
|
f3297 Location: |
Variable Format: character Notes: UNF:6:CE83FurSKiZWKCSKH8dIfg== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:iphnr8GamVPVrkc786WpZA== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:Tl1tEiiWwFgK6yMp0Hv3Vw== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:yYWIHWlBV6amvG7Yd9BhiQ== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:VEqyrZ8/k6tbnWzzY9SZ7A== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:rNLVFr8RvZmzieCtguObbw== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:pzk/HLZ0iXs0zn55GT/p+A== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:hieHIQz0Dc4HcQTT6zGdlg== |
|
f3297 Location: |
Variable Format: character Notes: UNF:6:a16F/onBvL5SY5NIn9kg5A== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:2b80Qr9GHUunlMDYpBKmlg== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:JkHEJYMXbNeQ8gZlT9du/g== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:Ixm8c5ektNfFpDhggJNJhQ== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:X2kq3cOvi9hKE1+5ccie2Q== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:9vn/mY1rx8N0SUE/HoomqQ== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:biMAvXeTjvoIV8aF2SsPPA== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:0v5KuEMT+bAzuyGf7yxmjw== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:DLKlptfjGj3e6tb7Szzz7g== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:BwlwTnj1VLb0D4H+TR5NVQ== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:dXe7BPwq/ZJvB6h1BW9T+g== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:FX/emqwUzAOrbF1pK64FNA== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:RmNHcHppi3kP8rbzezd5gg== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:Pe6miuFwMXya311B0e9G5g== |
|
f3153 Location: |
Variable Format: character Notes: UNF:6:4l3ZqrQE0i3pqjYg3K2U1w== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:AV1NyqBNYGDt9TH8dYWrKw== |
|
f3154 Location: |
Summary Statistics: StDev 0.7298382755284031; Mean -0.38012580336977386; Max. 4.83683; Valid 2918.0; Min. -6.60908 Variable Format: numeric Notes: UNF:6:4zNe2HZvzbdhwkEZifB2MQ== |
|
f3154 Location: |
Summary Statistics: Max. 1.74149; Min. -1.75015; Mean -0.22106292122374; StDev 0.39561945432237666; Valid 2221.0 Variable Format: numeric Notes: UNF:6:FKMWMXCbGrQdTqOjbiGOrA== |
|
f3154 Location: |
Summary Statistics: Min. 2.0; StDev 682.2369037061802; Max. 6753.0; Mean 544.356408498969; Valid 2918.0 Variable Format: numeric Notes: UNF:6:DF+MkqHsiSvKO1KIShKieQ== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:lXKkew6Q7CjwLW2i81wT2g== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:zrCjQMh1de1cxQJAU1MLIw== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:Dm0J1omjL5BfrZXca6q25w== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:n8e8XwijErFqcKcbSXIsvw== |
|
f3154 Location: |
Variable Format: character Notes: UNF:6:zySxQA5suzwMNIEk/ms1eg== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:iEPuokut3e4/Kxc6z04EqQ== |
|
f3155 Location: |
Summary Statistics: Min. -2.42166765492975; Mean -0.15264262371667983; StDev 0.7855580404106924; Max. 5.58755012944285; Valid 223.0; Variable Format: numeric Notes: UNF:6:J5Bafkl8GkKu90wrVyHS8w== |
|
f3155 Location: |
Summary Statistics: Min. -1.15276751926177; Valid 93.0; Mean 0.047936784842966165; StDev 0.24921960581747996; Max. 0.573724509002663; Variable Format: numeric Notes: UNF:6:09OqScMnE04MrUuLTjv/Nw== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:Wm6JpD4NNu1eTQHZH7GZcA== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:RCLN5dAby7+2zG2nQhnT6Q== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:OJP/LspnxE0YXjpJNdWffw== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:4qnemttR4QrAwHhGo5L1zw== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:46VsFgf7CABeESorIQT4Ew== |
|
f3155 Location: |
Variable Format: character Notes: UNF:6:WVOWaRgKMRpYq8KTDeh1ng== |