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Part 1: Document Description
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Citation |
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Title: |
Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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Identification Number: |
doi:10.11588/data/AOSUS8 |
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Distributor: |
heiDATA |
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Date of Distribution: |
2021-07-06 |
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Version: |
1 |
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Bibliographic Citation: |
Zilova, Lucie; Weinhardt, Venera; Tavhelidse, Tinatini; Schlagheck, Christina; Thumberger, Thomas; Wittbrodt, Joachim, 2021, "Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation", https://doi.org/10.11588/data/AOSUS8, heiDATA, V1 |
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Citation |
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Title: |
Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development - in vivo imaging of OV formation |
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Subtitle: |
In vivo time-lapse images acquired with MuVi SPIM of optic vesicle-like structure evagination in Rx3::H2B-GFP retinal organoids |
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Identification Number: |
doi:10.11588/data/AOSUS8 |
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Authoring Entity: |
Zilova, Lucie (Heidelberg University, Centre of Organismic Studies) |
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Weinhardt, Venera (Heidelberg University, Centre of Organismic Studies) |
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Tavhelidse, Tinatini (Heidelberg University, Centre of Organismic Studies) |
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Schlagheck, Christina (Heidelberg University, Centre of Organismic Studies, Heidelberg International Biosciences Graduate School HBIGS and HeiKa Graduate School on “Functional Materials”) |
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Thumberger, Thomas (Heidelberg University, Centre of Organismic Studies) |
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Wittbrodt, Joachim (Heidelberg University, Centre of Organismic Studies) |
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Grant Number: |
Synergy Grant IndiGene 810172 |
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Grant Number: |
Excellence cluster 3D Matter Made to Order |
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Grant Number: |
WE 6221/2-1 |
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Grant Number: |
HeiKa Graduate School |
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Distributor: |
heiDATA |
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Access Authority: |
Weinhardt, Venera |
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Date of Deposit: |
2021-06-17 |
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Holdings Information: |
https://doi.org/10.11588/data/AOSUS8 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, light-sheet microscopy, in vivo imaging, organoid, retina, medaka fish |
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Abstract: |
Development of a functional retina is accompanied by several morphological events transitions that ultimately result in the formation of a functional retina. One of them is the formation of the OV. When fish-derived organoids were generated with reduced cell-seeding density by 50%, the organoids formed individual domains reminiscent of the forming optic vesicle in vivo. To address the mode of OV formation in fish organoids, we employed live imaging of Rx3::H2B-GFP-derived organoids from day 1 to day 2 of development. This dataset is a raw data from live imaging of Rx3::H2B-GFP-derived organoids from day 1 to day 2 of development performed on the 16× detection MuVi SPIM Multiview light-sheet microscope (Luxendo Light-sheet, Bruker Corporation). |
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Kind of Data: |
imaging data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
https://doi.org/10.7554/eLife.66998 https://doi.org/10.1101/2021.01.28.428593 |
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Bibliographic Citation: |
https://doi.org/10.7554/eLife.66998 https://doi.org/10.1101/2021.01.28.428593 |
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