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Part 1: Document Description
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Citation |
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Title: |
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast [Dataset] |
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Identification Number: |
doi:10.11588/data/L45TRX |
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Distributor: |
heiDATA |
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Date of Distribution: |
2019-05-16 |
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Version: |
2 |
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Bibliographic Citation: |
Buchmuller, Benjamin C; Herbst, Konrad; Meurer, Matthias; Kirrmaier, Daniel; Sass, Ehud; Levy, Emmanuel D; Knop, Michael, 2019, "Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast [Dataset]", https://doi.org/10.11588/data/L45TRX, heiDATA, V2 |
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Citation |
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Title: |
Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast [Dataset] |
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Identification Number: |
doi:10.11588/data/L45TRX |
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Authoring Entity: |
Buchmuller, Benjamin C (Center for Molecular Biology of Heidelberg University (ZMBH)) |
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Herbst, Konrad (Center for Molecular Biology of Heidelberg University (ZMBH)) |
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Meurer, Matthias (Center for Molecular Biology of Heidelberg University (ZMBH)) |
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Kirrmaier, Daniel (Center for Molecular Biology of Heidelberg University (ZMBH)) |
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Sass, Ehud (Department of Structural Biology, Weizmann Institute of Science) |
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Levy, Emmanuel D (Department of Structural Biology, Weizmann Institute of Science) |
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Knop, Michael (Center for Molecular Biology of Heidelberg University (ZMBH)) |
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Date of Production: |
2019 |
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Distributor: |
heiDATA |
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Access Authority: |
Prof. Dr. Michael Knop |
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Date of Deposit: |
2019-05-15 |
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Holdings Information: |
https://doi.org/10.11588/data/L45TRX |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, CRISPR-assisted genome engineering, targeted nex-generation sequencing |
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Abstract: |
Data accompanying the paper "Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast" by Buchmuller and Herbst et al, 2019, Nat Communications. This contains raw NGS data for all genotyping analysis in the publication as well as the source code of the castR design software (v1.0). |
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Time Period: |
2017-2018 |
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Kind of Data: |
next-generation sequencing analysis |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Buchmuller, B.C., Herbst, K., Meurer, M. et al. Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast. Nat Commun 10, 2960 (2019). |
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Identification Number: |
https://doi.org/10.1038/s41467-019-10816-7 |
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Bibliographic Citation: |
Buchmuller, B.C., Herbst, K., Meurer, M. et al. Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast. Nat Commun 10, 2960 (2019). |
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Label: |
CASTLING_rawdata_201704-illumina_poolC_PCR.zip |
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Text: |
TruSeq amplicon sequencing of 1x sampled PCR |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201706-illumina_poolC_PCR.zip |
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Text: |
TruSeq amplicon sequencing of 10x sampled PCR |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201706-illumina_poolC_RCA.zip |
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Text: |
TruSeq amplicon sequencing of RCA |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201708-illumina_poolC_libs.zip |
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Text: |
multiplexed Anchor-Seq (CDS-tag junction) of libraries (#1.1, #1.2, #1.3), one unspecified lib (data not shown) and the FACS enriched (1st FACS) lib #1.1 |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201709-minion_poolC_libs_mNeonGreen_phenobins.zip |
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Text: |
multiplexed Anchor-Seq (CDS-tag junction) of FACS binning (2nd FACS) of FACS enriched (1st FACS) lib #1.1 |
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Notes: |
application/zip |
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CASTLING_rawdata_201805-illumina_poolAB1B2_PCR_RCA.zip |
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Text: |
multiplexed TruSeq amplicon sequencing of PCR and RCA for libraries 1a, 1b, 2a, 2b, 3a, 3b, 4a, 4b (only R2 was used for analysis) |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201806-illumina_poolAB1B2_libs.zip |
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Text: |
multiplexed Anchor-Seq (tag-3'UTR junction) of libraries 1a, 1b, 2a, 2b, 3a, 3b, 4a, 4b |
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Notes: |
application/zip |
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Label: |
CASTLING_rawdata_201806-illumina_poolD_libs.zip |
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Text: |
multiplexed Anchor-Seq (tag-3'UTR junction) of libraries LibA, LibB, LibC (in contrast to LibA and LibB, LibC was sequenced in the same run as libraries 1a-4b) |
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Notes: |
application/zip |
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Label: |
castR-1.0.zip |
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Text: |
Sourcecode Designsoftware of Pooled Genome-Wide Tag Libraries v1.0 (also https://github.com/knoplab/castR/tree/v1.0) |
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Notes: |
application/zip |
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Label: |
README-CASTLING_NGS_rawdata.txt |
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Text: |
description of raw sequencing data (individual .zip files) |
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Notes: |
text/plain |