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Part 1: Document Description
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Citation |
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Title: |
Switchable Zinc (II)-Responsive Globular β-Sheet Peptide [Research Data] |
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Identification Number: |
doi:10.11588/data/HV4MJW |
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Distributor: |
heiDATA |
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Date of Distribution: |
2022-01-19 |
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Version: |
1 |
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Bibliographic Citation: |
Pham, Truc Lam; Kovermann, Michael; Thomas, Franziska, 2022, "Switchable Zinc (II)-Responsive Globular β-Sheet Peptide [Research Data]", https://doi.org/10.11588/data/HV4MJW, heiDATA, V1 |
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Citation |
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Title: |
Switchable Zinc (II)-Responsive Globular β-Sheet Peptide [Research Data] |
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Identification Number: |
doi:10.11588/data/HV4MJW |
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Authoring Entity: |
Pham, Truc Lam (Heidelberg University, Institute of Organic Chemistry) |
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Kovermann, Michael (University of Konstanz, Department of Chemistry) |
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Thomas, Franziska (Heidelberg University, Institute of Organic Chemistry) |
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Grant Number: |
414261058, 2082/1–390761711 (3DMM2O) |
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Grant Number: |
Kekulé Fellowship (T.L.P) |
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Grant Number: |
(M.K) |
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Distributor: |
heiDATA |
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Access Authority: |
Thomas, Franziska |
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Holdings Information: |
https://doi.org/10.11588/data/HV4MJW |
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Study Scope |
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Keywords: |
Chemistry, Engineering, Other, pH-switch, protein engineering, receptor, WW domain, zinc switch |
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Abstract: |
The natural function of many proteins depends on their ability to switch their conformation driven by environmental changes. In this work, we present a small, monomeric β-sheet peptide that switches between a molten globule and a folded state through Zn(II) binding. The solvent-exposed hydrophobic core on the β-sheet surface was substituted by a His3-site, whereas the internal hydrophobic core was left intact. Zn(II) is specifically recognized by the peptide relative to other divalent metal ions, binds in the lower micromolar range, and can be removed and re-added without denaturation of the peptide. In addition, the peptide is fully pH-switchable, has a pKa of about 6, and survives several cycles of acidification and neutralization. In-depth structural characterization of the switch was achieved by concerted application of circular dichroism (CD) and multinuclear NMR spectroscopy. Thus, this study represents a viable approach toward a globular β-sheet Zn(II) mini-receptor prototype. |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Pham, T. L., Kovermann, M., & Thomas, F. (2022). Switchable Zinc(II)-Responsive Globular β-Sheet Peptide. ACS Synthetic Biology, 11, 1, 254–264. |
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Identification Number: |
10.1021/acssynbio.1c00396 |
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Bibliographic Citation: |
Pham, T. L., Kovermann, M., & Thomas, F. (2022). Switchable Zinc(II)-Responsive Globular β-Sheet Peptide. ACS Synthetic Biology, 11, 1, 254–264. |
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Label: |
1_CD-Spectroscopy.zip |
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Notes: |
application/zip |
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Label: |
2_NMR-Spectroscopy.zip |
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Notes: |
application/zip |
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Label: |
3_HPLC.zip |
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Notes: |
application/zip |
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4_MALDI-TOF-MS.zip |
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Notes: |
application/zip |
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Label: |
Overview_Original_Data.pdf |
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Notes: |
application/pdf |