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Part 1: Document Description
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Citation |
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Title: |
Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset] |
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Identification Number: |
doi:10.11588/data/N8NHE2 |
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Distributor: |
heiDATA |
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Date of Distribution: |
2017-12-04 |
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Version: |
1 |
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Bibliographic Citation: |
Hildenbrand, Georg; Metzler, Philipp; Pilarczyk, Goetz; Bobu, Vladimir; Hosser, Hiltraud; Fleckenstein, Jens; Krufczik, Matthias; Bestvater, Felix; Wenz, Frederik; Hausmann, Michael, 2017, "Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset]", https://doi.org/10.11588/data/N8NHE2, heiDATA, V1 |
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Citation |
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Title: |
Dose Enhancement Effects of Gold Nanoparticles Specifically Targeting RNA in Breast Cancer Cells [Dataset] |
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Identification Number: |
doi:10.11588/data/N8NHE2 |
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Authoring Entity: |
Hildenbrand, Georg (Department of Radiation Oncology, University Medical Centre Mannheim) |
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Metzler, Philipp (Kirchhoff-Institute for Physics, Heidelberg University) |
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Pilarczyk, Goetz (Kirchhoff-Institute for Physics, Heidelberg University) |
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Bobu, Vladimir (Department of Radiation Oncology, University Medical Centre Mannheim) |
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Hosser, Hiltraud (Department of Radiation Oncology, University Medical Centre Mannheim) |
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Fleckenstein, Jens (Department of Radiation Oncology, University Medical Centre Mannheim) |
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Krufczik, Matthias (Kirchhoff-Institute for Physics, Heidelberg University) |
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Bestvater, Felix (German Cancer Research Center (DKFZ), Heidelberg) |
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Wenz, Frederik (Department of Radiation Oncology, University Medical Centre Mannheim) |
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Hausmann, Michael (Kirchhoff-Institute for Physics, Heidelberg University) |
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Distributor: |
heiDATA |
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Access Authority: |
Hausmann, Michael |
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Date of Deposit: |
2017-11-29 |
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Holdings Information: |
https://doi.org/10.11588/data/N8NHE2 |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Physics |
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Abstract: |
Localization microscopy has shown to be capable of systematic investigations on the arrangement and counting of cellular uptake of gold nanoparticles (GNP) with nanometer resolution. In this article, we show that the application of specially modified RNA targeting gold nanoparticles (“SmartFlares”) can result in ring like shaped GNP arrangements around the cell nucleus. Transmission electron microscopy revealed GNP accumulation in vicinity to the intracellular membrane structures including them of the endoplasmatic reticulum. A quantification of the radio therapeutic dose enhancement as a proof of principle was conducted with γH2AX foci analysis: The application of both – SmartFlares and unmodified GNPs – lead to a significant dose enhancement with a factor of up to 1.2 times the dose deposition compared to non-treated breast cancer cells. This enhancement effect was even more pronounced for SmartFlares. Furthermore, it was shown that a magnetic field of 1 Tesla simultaneously applied during irradiation has no detectable influence on neither the structure nor the dose enhancement dealt by gold nanoparticles. |
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Notes: |
.nd2-files can be read e.g. via <a href="https://www.nikoninstruments.com/en_DE/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer">NIS-Elements Viewer</a> |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Title: |
Hildenbrand G, Metzler P, Pilarczyk G, Bobu V, Kriz W, et al. (2018) Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells. PLOS ONE 13(1): e0190183. |
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Identification Number: |
10.1371/journal.pone.0190183 |
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Bibliographic Citation: |
Hildenbrand G, Metzler P, Pilarczyk G, Bobu V, Kriz W, et al. (2018) Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells. PLOS ONE 13(1): e0190183. |
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Citation |
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Title: |
Moser F, Hildenbrand G, Müller P, Al Saroori A, Biswas A, Bach M, Wenz F, Cremer C, Burger N, Veldwijk M, Hausmann M (2016) Cellular uptake of gold nanoparticles and their behavior as labels for localization microscopy. Biophys J 110: 947-953 |
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Identification Number: |
10.1016/j.bpj.2016.01.004 |
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Bibliographic Citation: |
Moser F, Hildenbrand G, Müller P, Al Saroori A, Biswas A, Bach M, Wenz F, Cremer C, Burger N, Veldwijk M, Hausmann M (2016) Cellular uptake of gold nanoparticles and their behavior as labels for localization microscopy. Biophys J 110: 947-953 |
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Citation |
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Title: |
Burger N, Biswas A, Barzan D, Kirchner A, Hosser H, Hausmann M, Hildenbrand G, Herskind C, Wenz F, Veldwijk MR (2014) A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells. Nanomedicine: NBM 10: 1365-1373 |
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Identification Number: |
10.1016/j.nano.2014.03.011 |
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Bibliographic Citation: |
Burger N, Biswas A, Barzan D, Kirchner A, Hosser H, Hausmann M, Hildenbrand G, Herskind C, Wenz F, Veldwijk MR (2014) A method for the efficient cellular uptake and retention of small modified gold nanoparticles for the radiosensitization of cells. Nanomedicine: NBM 10: 1365-1373 |
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Label: |
Foci_Analysis.zip |
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Text: |
nd2-files [can be read e.g. via NIS-Elements Viewer (https://www.nikoninstruments.com/en_DE/Products/Software/NIS-Elements-Advanced-Research/NIS-Elements-Viewer)] |
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Notes: |
application/zip |
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Label: |
Localization_Microscopy.zip |
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Text: |
png files |
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Notes: |
application/zip |